ABSTRACT
O artigo discute a complexidade da Biossegurança na análise do risco de doenças infecciosas, apontando medidas de Biossegurança frente ao risco dos agentes etiológicos envolvidos. lizaram-se os dados sobre as zoonoses e doenças transmitidas por vetores notificadas pela Vigilância Epidemiológica, da Secretaria de Saúde de Rio Bonito (RJ), ocorridas no período de 2007 a 2008. Houve a predominância de agravos cujos agentes são da classe de risco 2, porém há também os de classe de risco 3. Demonstra-se a relação entre o potencial de risco, a pertinência de investimentos em Biossegurança, na capacitação de recursos humanos, a partir do contexto epidemiológico traduzido pelo sistema de notificação.
The study aims To discuss the complexity of the Biosafety in the risk analysis of infectious diseases, point out the Biosafety measures to face biological agents involved. Data on main zoonotic and vector borne diseases identified in the period of 2007 to 2008 through the surveillance of information obtained at the Rio Bonito County Secretary of Health was used. The greatest numbers of cases notified and collected whose agents are risk group 2, however also has events, in which the agents are risk group 3. In conclusion, from the analysis of epidemiologic relevance of collected and notified events and their relation to latent or manifested risk potential, funding issues or investments in Biosafety are indeed relevant, especially in capacity-building within the epidemiologic context expressed by the notification system.
ABSTRACT
The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3 percent sensitivity, 93.3 percent specificity, a 99.2 percent positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.